: Reproduction: nuclear transfer

nuclear transfer

Nuclear transfer, as opposed to blastomere separation and culture or embryo splitting, is the technique of choice for the production of clonally derived mammals because clone size can be unlimited given the availability of nuclear donor cells that can be propagated and maintained in vitro. Individual steps in the nuclear transfer procedure include oocyte recovery and maturation from excised ovaries, or ovarian stimulation and the recovery of MII oocytes; enucleation and preparation of the cytoplast (chromosome removal by micromanipulation); donor nucleus isolation and transfer to the cytoplast to produce an unfused pair; chemical activation of the cytoplast; electrically induced fusion of unfused pairs; embryo culture; and finally, embryo transfer to the oviduct or uterus of a synchronized recipient either with or without prior embryo cryopreservation and low-temperature storage. The oocyte must reprogram the nucleus, a task that before 1997 was thought to be impossible if the donor cell originated from a highly differentiated tissue. (Source : INRA)

https://opendata.inra.fr/BRMH/210

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