Concept information
Preferred term
sperm sorting
Entry terms
- flow cytometric sorting of sperm
- flow cytometric sorting of X and Y-chromosome bearing sperm
- flow cytometric sperm sorting
- semen sexing
- sperm separation
Note
- In mammals, the only known method of reliably separating X- and Y-bearing spermatozoa in order to produce offspring of a specific sex is flow cytometric sorting. This method is based on the observation that X-chromosome bearing spermatozoa of large farm animals (i.e. cow, sheep, pig and horse) contain 3.5-4.2% more DNA than Y-bearing spermatozoa. In this method relative DNA content is determined by quantitative staining with Hoechst 33342 and DNA content is measured using a modified cell sorter. The major constraint on widespread application of this technique has been the slow sorting rate. In the case of the human, the DNA difference is around 2.8%. Fluorescence in-situ hybridization (FISH) has confirmed sorting accuracy. (Source : INRA)
- spermatozoa is the plural form of spermatozoon. (Source : INRA)
Belongs to group
Subject
- Genetics and heredity
- Reproduction biology
preferred label (skos-xl)
- https://opendata.inra.fr/BRMH/xl_fr_220-1 (Reproduction)
In other languages
-
tri des spermatozoïdes X et Ylexinfo:gender: masculin
lexinfo:partOfSpeech: noun
French
-
sexage des spermatozoïdes
URI
https://opendata.inra.fr/BRMH/220
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