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Concept information

analysis/measurement > diagnosis method > quantitative polymerase chain reaction

Preferred term

quantitative polymerase chain reaction  

Definition

  • A real-time polymerase chain reaction (real-time PCR) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (quantitative real-time PCR) and semi-quantitatively (i.e., above/below a certain amount of DNA molecules) (semi-quantitative real-time PCR).

Broader concept

Entry terms

  • real-time polymerase chain reaction
  • rt-pcr

In other languages

URI

http://data.loterre.fr/ark:/67375/INS-TXMPC81C-0

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