: MeSH: Two-Hybrid System Techniques

Investigative Techniques > Genetic Techniques > Cloning, Molecular > Two-Hybrid System Techniques

Investigative Techniques > Molecular Probe Techniques > Protein Interaction Mapping > Two-Hybrid System Techniques

Two-Hybrid System Techniques

Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions. Variations on the two-hybrid system technique that analyze for proteins interacting with specific DNA sequences. Variations on the two-hybrid system technique that analyze for mutations or small molecules that interfere with or dissociate specific protein-DNA interactions. Variations on the two-hybrid system technique that analyze for mutations or small molecules that interfere with or dissociate specific protein-protein interactions. Variations on the two-hybrid system technique that analyze for proteins that interact with specific RNA molecules. Variations on the two-hybrid system technique. Variations on the two-hybrid system technique that analyze for mutations or small molecules that interfere with or dissociate specific interactions between protein and RNA molecules or other ligands.

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