: MeSH: Enzyme Assays

Investigative Techniques > Chemistry Techniques, Analytical > Enzyme Assays

Enzyme Assays

Methods used to measure the relative activity of a specific enzyme or its concentration in solution. Typically an enzyme substrate is added to a buffer solution containing enzyme and the rate of conversion of substrate to product is measured under controlled conditions. Many classical enzymatic assay methods involve the use of synthetic colorimetric substrates and measuring the reaction rates using a spectrophotometer. Sensitive detection methods using enzymes to indirectly measure substances that would normally be difficult to quantitate.

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