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... > Diagnosis > Diagnostic Techniques and Procedures > Diagnostic Imaging > Microscopy > Microscopy, Fluorescence > Microscopy, Fluorescence, Multiphoton
Investigative Techniques > Microscopy > Microscopy, Fluorescence > Microscopy, Fluorescence, Multiphoton
... > Natural Science Disciplines > Physics > Optics and Photonics > Microscopy > Microscopy, Fluorescence > Microscopy, Fluorescence, Multiphoton
... > Technology, Industry, and Agriculture > Engineering > Optics and Photonics > Microscopy > Microscopy, Fluorescence > Microscopy, Fluorescence, Multiphoton
... > Diagnosis > Diagnostic Techniques and Procedures > Diagnostic Imaging > Microscopy > Nonlinear Optical Microscopy > Microscopy, Fluorescence, Multiphoton
Investigative Techniques > Microscopy > Nonlinear Optical Microscopy > Microscopy, Fluorescence, Multiphoton
... > Natural Science Disciplines > Physics > Optics and Photonics > Microscopy > Nonlinear Optical Microscopy > Microscopy, Fluorescence, Multiphoton
... > Technology, Industry, and Agriculture > Engineering > Optics and Photonics > Microscopy > Nonlinear Optical Microscopy > Microscopy, Fluorescence, Multiphoton

Preferred term

Microscopy, Fluorescence, Multiphoton  

Type

  • mesh:Descriptor

Definition

  • Fluorescence microscopy utilizing multiple low-energy photons to produce the excitation event of the fluorophore (endogenous fluorescent molecules in living tissues or FLUORESCENT DYES). Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal confocal microscopes. Ultimately this allows spatial isolation of the excitation event, enabling deeper imaging into optically thick tissue, while restricting photobleaching and phototoxicity to the area being imaged.

Broader concept

Entry terms

  • Fluorescence Microscopy, Multiphoton
  • Multiphoton Excitation Microscopy
  • Multiphoton Fluorescence Microscopy

Allowable Qualifier(s)

In other languages

URI

http://data.loterre.fr/ark:/67375/JVR-RRXH4CX3-9

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RDF/XML TURTLE JSON-LD Created 7/3/02, last modified 6/30/17