Concept information
Preferred term
Tandem Affinity Purification
Type
-
mesh:Descriptor
Definition
- A technique used to isolate specific interacting partners of MACROMOLECULAR COMPLEXES that have been assembled with a tagged component (bait) that interacts with other partners in the complex. The interacting bait component is labeled with a dual-affinity tag that allows for capture of the complex by IMMUNOPRECIPITATION, followed by purification of only the partners of the complex interacting with the bait by affinity chromatography.
Broader concept
Allowable Qualifier(s)
In other languages
URI
http://data.loterre.fr/ark:/67375/JVR-SB55QZSK-7
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