: MeSH: Complement Fixation Tests

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Investigative Techniques > Clinical Laboratory Techniques > Immunologic Tests > Serologic Tests > Complement Fixation Tests

Investigative Techniques > Immunologic Techniques > Immunologic Tests > Serologic Tests > Complement Fixation Tests

Complement Fixation Tests

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Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.

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