Concept information
Término preferido
Chromatin Immunoprecipitation
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Tipo
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mesh:Descriptor
Definición
- A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.
Concepto genérico
Conceptos específicos
Etiquetas alternativas
- Immunoprecipitation, Chromatin
En otras lenguas
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francés
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Immuno-précipitation de chromatine
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Immuno-précipitation de la chromatine
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Immunoprécipitation de chromatine
URI
http://data.loterre.fr/ark:/67375/JVR-GW73DX76-6
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