: MeSH: Microspectrophotometry

Investigative Techniques > Chemistry Techniques, Analytical > Spectrum Analysis > Spectrophotometry > Microspectrophotometry

Investigative Techniques > Chemistry Techniques, Analytical > Photometry > Spectrophotometry > Microspectrophotometry

Microspectrophotometry

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Analytical technique for studying substances present at enzyme concentrations in single cells, in situ, by measuring light absorption. Light from a tungsten strip lamp or xenon arc dispersed by a grating monochromator illuminates the optical system of a microscope. The absorbance of light is measured (in nanometers) by comparing the difference between the image of the sample and a reference image.

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