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Tandem Affinity Purification  

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Tipo

  • mesh:Descriptor

Definición

  • A technique used to isolate specific interacting partners of MACROMOLECULAR COMPLEXES that have been assembled with a tagged component (bait) that interacts with other partners in the complex. The interacting bait component is labeled with a dual-affinity tag that allows for capture of the complex by IMMUNOPRECIPITATION, followed by purification of only the partners of the complex interacting with the bait by affinity chromatography.

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URI

http://data.loterre.fr/ark:/67375/JVR-SB55QZSK-7

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