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... > Diagnosis > Diagnostic Techniques and Procedures > Clinical Laboratory Techniques > Histological Techniques > Histocytological Preparation Techniques > Replica Techniques > Freeze Fracturing > Freeze Etching
... > Investigative Techniques > Clinical Laboratory Techniques > Histological Techniques > Histocytological Preparation Techniques > Replica Techniques > Freeze Fracturing > Freeze Etching
... > Diagnosis > Diagnostic Techniques and Procedures > Clinical Laboratory Techniques > Cytological Techniques > Histocytological Preparation Techniques > Replica Techniques > Freeze Fracturing > Freeze Etching
... > Investigative Techniques > Clinical Laboratory Techniques > Cytological Techniques > Histocytological Preparation Techniques > Replica Techniques > Freeze Fracturing > Freeze Etching

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Freeze Etching  

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Tipo

  • mesh:Descriptor

Definición

  • A replica technique in which cells are frozen to a very low temperature and cracked with a knife blade to expose the interior surfaces of the cells or cell membranes. The cracked cell surfaces are then freeze-dried to expose their constituents. The surfaces are now ready for shadowing to be viewed using an electron microscope. This method differs from freeze-fracturing in that no cryoprotectant is used and, thus, allows for the sublimation of water during the freeze-drying process to etch the surfaces.

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http://data.loterre.fr/ark:/67375/JVR-WQPGKL8F-3

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