Concept information
Terme préférentiel
Chromatin Immunoprecipitation
Type
-
mesh:Descriptor
Définition
- A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.
Concept générique
Concepts spécifiques
Synonyme(s)
- Immunoprecipitation, Chromatin
Qualificatif(s) autorisé(s)
- adverse effects (Qualifier)
- classification (Qualifier)
- economics (Qualifier)
- ethics (Qualifier)
- history (Qualifier)
- instrumentation (Qualifier)
- methods (Qualifier)
- mortality (Qualifier)
- nursing (Qualifier)
- psychology (Qualifier)
- standards (Qualifier)
- statistics & numerical data (Qualifier)
- trends (Qualifier)
- veterinary (Qualifier)
Traductions
-
français
-
Immuno-précipitation de chromatine
-
Immuno-précipitation de la chromatine
-
Immunoprécipitation de chromatine
URI
http://data.loterre.fr/ark:/67375/JVR-GW73DX76-6
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