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Concept information

Investigative Techniques > Genetic Techniques > Nucleic Acid Amplification Techniques > Polymerase Chain Reaction

Terme préférentiel

Polymerase Chain Reaction  

Type

  • mesh:Descriptor

Définition

  • In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.

Concept générique

Concepts spécifiques

Concepts associés

Synonyme(s)

  • PCR

Qualificatif(s) autorisé(s)

Traductions

URI

http://data.loterre.fr/ark:/67375/JVR-XS88F8W1-Q

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RDF/XML TURTLE JSON-LD Date de création 11/01/1990, dernière modification le 24/06/2011